Effects of Brusatol treatment on oxidative stress and mitochondrion.

<p>A) Brusatol reduced the mRNA level of Nrf2 target genes. oocytes were treated with 200 nM of brusatol for 4h, 8h, 14h and subjected to qRT-PCR. B) Representative images of CM-H2DCFDA fluorescence in control, and Brusatol treated oocytes to show the ROS levels. C) Quantitative analysis of fluorescence intensity shown in B. D) Brusatol reduced the mRNA level of mitochondrial related genes. E) To mark the mitochondrion, representative images of mito-tracker fluorescence in control, and Brusatol treated oocytes were taken. F) Quantitative analysis of fluorescence intensity shown in E. Scale bar, 20 μm. *p < 0.05.