Additional file 2: Figure S2. of Targeting the CXCR4 pathway using a novel anti-CXCR4 IgG1 antibody (PF-06747143) in chronic lymphocytic leukemia

PF-06747143 and its Fab and F(ab’)2 forms block CXCL12-induced calcium flux. The calcium flux assay was performed in human T cell leukemia Jurkat cells incubated with PF-06747143 full-length (FL), PF-06747143-Fab, PF-06747143 F(ab’)2, or isotype control IgG1 antibody in presence of CXCL12 at 8 nM. For adequate comparison between the different forms of the antibody, their concentrations were adjusted relative to their antigen-binding site numbers. Experiment was performed in quadruplicates. The mean intracellular calcium concentration is shown in relative fluorescence units (RFU). Bars denote standard error of the mean (SEM). (PDF 389 kb