Characterization of human AD-MSCs.

<p>(A) Morphological characteristics of MSCs five days after plating. (B) Representative images of AD-MSCs at passage three, differentiated towards adipogenic and osteogenic lineages. Lipids droplets were stained with Oil red-O whereas hydroxyapatite precipitates were stained with alizarin red. (C) Flow cytometry analysis of AD-MSCs at passage three, for putative human MSC markers (CD29, CD13, CD105, CD73 and CD90) and markers characteristic of other cell lineages (CD235a, CD31 and CD45). Grey histograms represent isotype controls while black histograms represent specific antibodies. Data shown are representative of MSCs isolated from four different donors. Experiments were repeated three times at technical level.