Proliferation and apoptosis profile of anti-fungal memory CD8⁺ T cells.

<p>Naïve IL17a^CreR26R^eYFP mice were depleted of CD4⁺ T cells and vaccinated as noted in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006356#ppat.1006356.g001" target="_blank">Fig 1</a>. <b>A.</b> Proliferation of anti-fungal effector cells, and early and late memory CD8⁺ T cells. Vaccinated mice were pulsed with BrdU in drinking water (DW) for 12 days. On the following day, spleens were harvested, processed and re-stimulated. Cells were surface-stained and stained intracellularly for cytokines before analysis of BrdU staining. Numbers represent the percent BrdU⁺ of eYFP⁺/IFNγ⁺ CD8⁺ T cells. N = 5 mice/group. <b>B.</b> On day 76 post-vaccination, surface-stained splenocytes were stained intracellularly for active Caspase3/8. Dot plots show the staining of pro-apoptotic caspases gated on CD8⁺CD44^hi T cells. N = 4–5 mice. <b>C.</b> On day 76 post-vaccination, splenocytes were stained for surface markers before staining for intracellular anti-apoptotic factors. Values indicate MFIs gated on CD8⁺CD44^hi eYFP^+/- T cells. Dotted lines indicate staining controls. Data are representative of two independent experiments. *P≤0.05, ***P≤0.001, and ****P≤0.0001.