Leptin decreased cell aggregation, and increased cell migration and invasion.

<p>MCF7 or MCF10AT1 cells in low serum media were treated for 14 days with 200 ng/ml leptin (LEPT) and compared to untreated controls (CTRL). (<b>a</b>) CTRL and (<b>b</b>) LEPT-treated MCF7 cells are morphologically different. <b>(c)</b> Effect on MCF7 cells by hanging drop cell aggregation assay. ***P<0.001 (n = 5 drops, Student’s t-test. Data shown is from one representative experiment of 3 independent experiments.) <b>(d)</b> Effect on invasion of MCF7 cells into matrigel. ***P<0.001 (n = 18–20 wells, Student’s t-test, data combined from 3 independent experiments). <b>(e) and (f)</b> Effect on migration rate of <b>(e)</b> MCF7 and <b>(f)</b> MCF10AT1 cells measured by wound healing scratch assay. ***P<0.001 (n = 17–24 wells, Student’s t-test, data combined from several independent experiments).