SIRT1 increases the transcriptional activity of RUNX2.

<p><b>A)</b> A RUNX2 luciferase reporter assay (p6OSE2) shows that overexpression (OE) of SIRT1 increases luciferase activity, while RNA-interference (RNAi) of SIRT1 decreases it (n = 4). <b>B)</b> Two specific SIRT1 activators, SRT1720 and SRT2183, also increase luciferase activity (n = 4). <b>C)</b> SIRT1 activator, SRT2183, leads to induction of endogenous RUNX2 target Bone Sialoprotien (BSP) in wildtype (vector) but not SIRT1 excised (Cre) cells, indicating that the stimulatory effects of SRT2183 on RUNX2 is SIRT1-dependent. The expression of Runx2 itself is unchanged. (n≥3, * p<.05; ** p<.01; *** p<.005).