SIRT1 interacts with RUNX2.

<p><b>A)</b> Tagged versions of SIRT1 and RUNX2 interact in 293T cells: FLAG-tagged SIRT1 is able to co-immunoprecipitate HA-tagged RUNX2, and vice versa. (WB: western blot; IP: immunoprecipitation) <b>B)</b> This interaction also exists at the endogenous level. Two different RUNX2 antibodies (Sigma and Abcam) co-immunoprecipitate SIRT1, but not closely related SIRT6 or abundantly expressed HSP90, in U2OS osteosarcoma cell lysates. The band below the RUNX2 band (in the Runx2 WB panel) represents heavy chain IgG. (AB: antibody).