Myelin from glial fibrillary acidic protein (GFAP)-SREBP cleavage-activating protein (SCAP) brains shows increased accumulation of dietary lipids.

<p>Lipid extracts of purified myelin of GFAP-SCAP brains (P42) were analyzed using liquid chromatography and mass spectrometry. <b>A)</b> Polar membrane lipid concentration and <b>(B)</b> sterol concentration per protein amount in GFAP-SCAP compared to wild-type (WT) myelin. GSL, glycosphingolipid; PI, phosphatidyl inositol; PE, phosphatidyl ethanolamine; PS, phosphatidyl serine; PC, phosphatidyl choline; SM, sphingomyelin. <b>C)</b> Fatty acid profile of phospholipids from purified myelin with the amount of different fatty acids species as the percentage of the total amount. Fatty acid species are depicted as “y:z,” with “y” representing the length of the fatty acid molecules and “z” representing the number of double bonds. Insert: depicted are proportions of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), and the ratio of 18:1/18:2. Data are presented as the mean percentage of WT ± SD. <i>t</i> tests: * = <i>p</i> < 0.05; ** = <i>p</i> < 0.01; *** = <i>p</i> < 0.001, <i>n</i> = 4. The numeric data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002605#pbio.1002605.s006" target="_blank">S1 Data</a>.