<p>(A) Intracellular staining was conducted to measure the expression of IL-17 and IFNr in CCR6<sup>+</sup> and CCR6<sup>−</sup> T cells and analyzed by FACS (B and C). The percentage of IL-17<sup>+</sup>IFNr<sup>−</sup> (B) and IFNr<sup>+</sup>IL-17<sup>−</sup> (C) cells were calculated. ***<i>P</i><0.001, ns: no significant difference. The data are representative of three independent experiments.</p
<p>CD4-positive lymphocytes were gated to analyze intracellular cytokine expressions by flow cytomet...
<p>Cells from spleen and small intestine LP were stimulated for four hours with PMA and ionomycin an...
<p>Spleen, draining lymph nodes and mesenteric lymph node were removed at 5<sup>th</sup> week after ...
<p>A. Representative flow cytometry analysis on antigen stimulated PBMC of one each of BT and LL pat...
<p>(<b>A</b>) The expression of IL-17A mRNA in Th17 cells treated with IFNβ. Naive T cells were cult...
<p>Intracellular staining for cytokine and transcription factors in CD4<sup>+</sup> T cells was perf...
<p>Cell numbers were assessed in ten independent fields at 400× magnification. Average cell numbers ...
<p>Splenic cells were isolated from C57BL/6 mice on day 7 after immunization and stimulated with NP ...
<p>(<i>A</i>) Representative flow cytometry images of Th17 (CD4<sup>+</sup> IL-17<sup>+</sup>) cells...
<p>(A) Isolated naïve CD4<sup>+</sup> T cells from WT control or <i>TCF-1<sup>-/-</sup></i> mice wer...
Cells were harvested on day 6 and restimulated with PMA plus ionomycin, followed by intracellular cy...
<p>(A) CD44, CD62L and CXCR6 expression on digested lymph node cells from control or <i>Cxcr6<sup>GF...
<p>(A) CD4<sup>+</sup> T cells from different coculturing groups were stained with specific antibodi...
<p>IFNγ and IL-17A producing CD4+ T cells were detected in the spleens and mLNs, 14 days after the s...
<p>Cells were isolated from either challenged only (c) or sensitized and challenged (s/c) <i>Il22</i...
<p>CD4-positive lymphocytes were gated to analyze intracellular cytokine expressions by flow cytomet...
<p>Cells from spleen and small intestine LP were stimulated for four hours with PMA and ionomycin an...
<p>Spleen, draining lymph nodes and mesenteric lymph node were removed at 5<sup>th</sup> week after ...
<p>A. Representative flow cytometry analysis on antigen stimulated PBMC of one each of BT and LL pat...
<p>(<b>A</b>) The expression of IL-17A mRNA in Th17 cells treated with IFNβ. Naive T cells were cult...
<p>Intracellular staining for cytokine and transcription factors in CD4<sup>+</sup> T cells was perf...
<p>Cell numbers were assessed in ten independent fields at 400× magnification. Average cell numbers ...
<p>Splenic cells were isolated from C57BL/6 mice on day 7 after immunization and stimulated with NP ...
<p>(<i>A</i>) Representative flow cytometry images of Th17 (CD4<sup>+</sup> IL-17<sup>+</sup>) cells...
<p>(A) Isolated naïve CD4<sup>+</sup> T cells from WT control or <i>TCF-1<sup>-/-</sup></i> mice wer...
Cells were harvested on day 6 and restimulated with PMA plus ionomycin, followed by intracellular cy...
<p>(A) CD44, CD62L and CXCR6 expression on digested lymph node cells from control or <i>Cxcr6<sup>GF...
<p>(A) CD4<sup>+</sup> T cells from different coculturing groups were stained with specific antibodi...
<p>IFNγ and IL-17A producing CD4+ T cells were detected in the spleens and mLNs, 14 days after the s...
<p>Cells were isolated from either challenged only (c) or sensitized and challenged (s/c) <i>Il22</i...
<p>CD4-positive lymphocytes were gated to analyze intracellular cytokine expressions by flow cytomet...
<p>Cells from spleen and small intestine LP were stimulated for four hours with PMA and ionomycin an...
<p>Spleen, draining lymph nodes and mesenteric lymph node were removed at 5<sup>th</sup> week after ...