VF-Fab from sera without detectable AAV shows neutralisation activity.

<p>VF-Fab1b-4-1, VF-Fab1b-6-1, VF-Fab1b-8-1 were purified from three unrelated patients infected with HCV genotype 1b that were negative for presence of AAV. HCVpp incorporating E1E2 derived from genotype 1a (HCVpp1b-1-3, HCVppH77) and 1b (HCVpp1b-1-2, HCVpp1b-1-3) were pre-incubated with different concentrations (0.006 to 0.4 mg/ml) of purified VF-Fabs prior to infection of Huh7 cells. A no envelope control was used to normalise the data. The neutralising activity of the VF-Fab is expressed as percentage of inhibition of the infectious titres. Each experiment was repeated three times. IC₅₀ for each VF-Fab is detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175349#pone.0175349.t003" target="_blank">Table 3</a>. Error bars indicate standard deviation.