Effects of TiO₂ nanoparticles on wheat (<i>Triticum aestivum L</i>.) seedlings cultivated under super-elevated and normal CO₂ conditions

<div><p>Concerns over the potential risks of nanomaterials to ecosystem have been raised, as it is highly possible that nanomaterials could be released to the environment and result in adverse effects on living organisms. Carbon dioxide (CO₂) is one of the main greenhouse gases. The level of CO₂ keeps increasing and subsequently causes a series of environmental problems, especially for agricultural crops. In the present study, we investigated the effects of TiO₂ NPs on wheat seedlings cultivated under super-elevated CO₂ conditions (5000 mg/L CO₂) and under normal CO₂ conditions (400 mg/L CO₂). Compared to the normal CO₂ condition, wheat grown under the elevated CO₂ condition showed increases of root biomass and large numbers of lateral roots. Under both CO₂ cultivation conditions, the abscisic acid (ABA) content in wheat seedlings increased with increasing concentrations of TiO₂ NPs. The indolepropioponic acid (IPA) and jasmonic acid (JA) content notably decreased in plants grown under super-elevated CO₂ conditions, while the JA content increased with increasing concentrations of TiO₂ NPs. Ti accumulation showed a dose-response manner in both wheat shoots and roots as TiO₂ NPs concentrations increased. Additionally, the presence of elevated CO₂ significantly promoted Ti accumulation and translocation in wheat treated with certain concentrations of TiO₂ NPs. This study will be of benefit to the understanding of the joint effects and physiological mechanism of high-CO₂ and nanoparticle to terrestrial plants.</p>