The silence efficiency of PTN siRNA measured by quantitative RT-PCR and western blotting.

<p><b>The PTN expression level showed a direct correlation with the proliferation rate of B16-F10 melanoma cells.</b> (A). Quantitative RT-PCR analysis of the knock-down efficiency of siRNA 1, siRNA 2 and the mixture of them at the same dose. GAPDH was used as a control. (B). Western blotting analysis of the knock-down effect of siRNA 1, siRNA 2 and the mixture of them at the same dose. β-actin was used as a control. The intensity of the control sample without transfection of any siRNA was set as 100%. Asterisks indicate statistically significant differences between samples (** p<0.01 and ns for no significant difference). (C). Silence of the PTN by siRNA reduced the proliferation rate of B16-F10 melanoma cells in a manner directly related the reduced level of PTN. Each point represents the mean ± standard deviation of 3 replicates.