Exogenous cyclic di-AMP recovers inflammasome activation in response to attenuated <i>C</i>. <i>trachomatis</i>.

<p>(A) IL-1β secretion analysed by ELISA in wild type BMDM in response to stimulation with gamma attenuated <i>C</i>. <i>trachomatis</i> (γ-CT) transfected with cyclic di-AMP (dcAMP) at indicated concentrations. Data represent the mean of BMDM obtained from six to nine individual mice, error bars indicate ±SEM. (B) IL-1β secretion analysed by ELISA of supernatants from wild-type BMDM following infection with CPAF deficient <i>C</i>. <i>trachomatis</i> (ΔCPAF) transfected with 10μM cyclic di-AMP (dcAMP) for 24hrs. Data represented as the mean of one experiment performed on cells from three individual mice, error bars indicate ±SEM. (C) IL-1β secretion analysed by ELISA in wild-type (STING^+/+) or STING deficient (STING^GT/GT) in response to stimulation with gamma attenuated <i>C</i>. <i>trachomatis</i> (γ-CT) transfected with cyclic di-AMP (dcAMP) (10μM). Data represent the mean of BMDM from three-individual mice, error bars indicate ±SEM *p = <0.05, **p = <0.01, ***p = <0.001.