PD-1 and SOCS3 mRNA expression of CD4+ T cells from tuberculosis patients and healthy contacts.

<p>The expression of PD-1 <b>(a)</b> and SOCS3 <b>(c)</b> was determined for mRNA isolated from CD4⁺ T cells, using Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a reference. Cycle threshold differences (2^-ΔCt) are shown for healthy contacts [n = 117 (PD-1), n = 119 (SOCS3)], and for tuberculosis patients prior to (0 months, n = 40), during (2 months, n = 28), and after (6 months, n = 17) treatment. Median and interquartile range is depicted. Spearman correlation between plasma IL-7 and <b>(b)</b> PD-1 or <b>(d)</b> SOCS3 for healthy contacts (circles) or tuberculosis patients (squares) prior to treatment. Each symbol indicates mean values of duplicates from each individual donor. Due to a low overlap between tuberculosis patients, exact Mann-Whitney U test used for comparison of all groups. Nominal p-values are indicated as * p < 0.05, ** p < 0.01, *** p < 0.001.