Additional file 2: Figure S2. of Tightly controlled MRTF-A activity regulates epithelial differentiation during formation of mammary acini

Characterization of MCF10A cells overexpressing MRTFs. a MRTF-A and MRTF-B protein expression in stably transduced pools of cells overexpressing MRTF-A or MRTF-B or vector-infected (mock) and parental control cells. A representative immunoblot with specific antibodies against MRTF-A, MRTF-B and GAPDH as a control is shown. Quantified relative protein amounts of MRTF-A were normalized to parental MCF10A cells. b Stably transduced cells were transiently transfected under serum-starved conditions, treated after 24 h with horse serum for 7 h if indicated and analyzed for MRTF/SRF reporter activity. Error bars SEM (n = 3): *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test). (TIFF 14057 kb