The effects of brain-derived neurotrophic factor (BDNF) on autoregulation, migration and migration related proteins signaling in MDA-MB-231 cells.

<p>MDA-MB-231 (4 X10⁵ / well) cells cultured with low serum medium were treated with BDNF (0-, 12,5-, 25-, and 50 ng/mL) for 24 h or 6 h, and analyzed with Western blot (A) or real-time PCR (B), respectively. TkrB FL and TrkB T1 indicated full length and T1 domain of TrkB, respectively. Cells at a density of 2×10⁴ cells were seeded into a 3.5 cm Petri dish containing an insert for migration assay (C) as described in Methods. Protein expressions of Rac, CDC42, and Rho (D) were quantified (E) and gene expression of COX2, MMP2, MMP9, and MMP13 were analyzed with Western blot (F,G) and real-time PCR (H). Data were expressed as mean + SEM. *, <i>p</i>< 0.05 compared to vehicle group by one way ANOVA, n = 4–5).