TRAIL expression in NDV-stimulated NK cells of mice where IFN-γ receptor one was deficient.

<p>(A) Purified splenic NK cells from IFN-R1^-/- mice were stimulated with NDV 7793 or IFN-γ. Cells were stained with a PE-conjugated monoclonal antibody (mAb) against TRAIL and then examined by flow cytometry. PBS-treatment NK cells were used as negative controls. Results are representative of three independent experiments. (B) Purified splenic NK cells from IFN-R1^+/+ and IFN-R1^-/- mice were stimulated with NDV 7793 or IFN-γ. Cellular proteins were extracted and subjected to western blotting using a mAb against TRAIL. Results are representative of three independent experiments. (C) The concentration of IFN-γ in supernatants was determined by ELISA. Values represent the mean + standard deviation from triplicate wells, with results obtained from three independent experiments. *<i>P</i><0.01 and ^Δ<i>P</i><0.01 <i>vs</i>. unstimulated NK cells in IFN-R1^+/+ and IFN-R1^-/- mice, respectively.