GI enhances TCP4-binding to the <i>CO</i> promoter.

<p>(A) The representative protein profiles of TCP4-3F6H overexpression in WT and <i>gi-2</i> background grown in LD are shown. Histone H3 protein was used as the loading control. The numbers above the images indicate time (h) after light onset within a day. (B) The quantified results of the TCP4-3F6H protein profiles shown in (A) are obtained from 3 independent biological replicates. Significant differences are indicated by asterisks (Student’s <i>t</i>-test, <i>p<</i>0.05). Data represent means ± SEM. (C) BiFC assay results of interactions among CIN-TCP proteins are shown. The full length of TCP2, 3, 5, 10, 13, 17, and 24 fused to YFP^c were co-expressed with YFPⁿ-mTCP4 in <i>N</i>. <i>benthamiana</i> leaf epidermal cells. Scale bar, 20 μm. (D) Results of ChIP analysis using <i>35S</i>:<i>TCP4-3F6H</i> in either WT and <i>gi-2</i> backgrounds harvested at ZT16 are shown. Amplicons located in the <i>CO</i> promoter are described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006856#pgen.1006856.g003" target="_blank">Fig 3D</a>. Significant differences are indicated by asterisks (Student’s <i>t</i>-test, <i>p<</i>0.05). Data represent means ± SEM (<i>n</i> = 4).