<p>T cells were characterized after 18h, in the acute and in the chronic model. They were identified as CD4<sup>+</sup> or CD8<sup>+</sup>, then CD103 expression on T cell subsets was verified in WT samples using <i>Cd103</i><sup><i>-/-</i></sup> mice as control for CD103 staining. Number of total lung <b>A)</b> CD4<sup>+</sup> T cells and <b>B)</b> CD8<sup>+</sup> T cells. <b>C)</b> Number and <b>D)</b> % of CD4/CD103<sup>+</sup> T cells. <b>E)</b> Number and <b>F)</b> % of CD8/CD103<sup>+</sup> T cells. For Tregs analysis CD25<sup>+</sup>Foxp3<sup>+</sup> on lung CD4<sup>+</sup> T cells was verified using FMO controls. <b>G)</b> % and <b>H)</b> number of Tregs. Results were obtained from 2 pooled experiments; n = 6–12 mice/group. * = p < ...
(A) Representative flow cytometry analysis of CD4+ Foxp3+ cells among CD3+ cells from spleen, lung, ...
<p>C57BL/6 and CBA/J mice were infected with an aerosolized dose of <i>Mtb</i> and at various timepo...
<p>(A) Purified SP4 thymocytes, and CD4<sup>+</sup> naïve T cells from lymph nodes of C57BL/6 mice w...
<p>A-D) Splenocytes or mucosal lymphocytes harvested from untreated Egr3WT or Egr3TG mice were count...
<p><b>(a)</b> The proportion of TB10Rg3 cells (%GFP<sup>+</sup>Vα2<sup>+</sup>) among CD8<sup>+</sup...
<p>CD4 or CD8 T cells were purified from <i>Socs4<sup>R108X/R108X</sup></i> or wild-type spleens by ...
<p>(Early: week 6; Intermediate: week 16, late: week 40) (A and B) Lung cells from infected mice wer...
<p>Single-cell suspensions prepared from lung tissues of mice infected with Mtb strains at 2, 5, 7, ...
<p>(A) Representative FACS plots demonstrating the gating strategy for CD4<sup>+</sup> T cells and T...
<p>(A) Cell numbers in pooled lymph nodes and spleen, d3. (B) T-cell subsets in lymph nodes. (C) Fre...
<p>Lymphocytes were isolated from WT and FtDKO mice. <i>A</i> and <i>B</i>, Representative FACS anal...
<p>The protocol of the experiment was as described in the legend of <a href="http://www.plospathogen...
<p>(A) The expression of KLRG-1 on the pooled spleen T cells of age-matched naïve, i.n. or s.c. BCG-...
<p>Lung cells were harvested and analyzed by flow cytometry on day 4 after adoptive transfer of 1x10...
<p>Cells from infected lungs were stimulated with TB10.4 <sub>3–11</sub> or TB10.4 <sub>74–88</sub> ...
(A) Representative flow cytometry analysis of CD4+ Foxp3+ cells among CD3+ cells from spleen, lung, ...
<p>C57BL/6 and CBA/J mice were infected with an aerosolized dose of <i>Mtb</i> and at various timepo...
<p>(A) Purified SP4 thymocytes, and CD4<sup>+</sup> naïve T cells from lymph nodes of C57BL/6 mice w...
<p>A-D) Splenocytes or mucosal lymphocytes harvested from untreated Egr3WT or Egr3TG mice were count...
<p><b>(a)</b> The proportion of TB10Rg3 cells (%GFP<sup>+</sup>Vα2<sup>+</sup>) among CD8<sup>+</sup...
<p>CD4 or CD8 T cells were purified from <i>Socs4<sup>R108X/R108X</sup></i> or wild-type spleens by ...
<p>(Early: week 6; Intermediate: week 16, late: week 40) (A and B) Lung cells from infected mice wer...
<p>Single-cell suspensions prepared from lung tissues of mice infected with Mtb strains at 2, 5, 7, ...
<p>(A) Representative FACS plots demonstrating the gating strategy for CD4<sup>+</sup> T cells and T...
<p>(A) Cell numbers in pooled lymph nodes and spleen, d3. (B) T-cell subsets in lymph nodes. (C) Fre...
<p>Lymphocytes were isolated from WT and FtDKO mice. <i>A</i> and <i>B</i>, Representative FACS anal...
<p>The protocol of the experiment was as described in the legend of <a href="http://www.plospathogen...
<p>(A) The expression of KLRG-1 on the pooled spleen T cells of age-matched naïve, i.n. or s.c. BCG-...
<p>Lung cells were harvested and analyzed by flow cytometry on day 4 after adoptive transfer of 1x10...
<p>Cells from infected lungs were stimulated with TB10.4 <sub>3–11</sub> or TB10.4 <sub>74–88</sub> ...
(A) Representative flow cytometry analysis of CD4+ Foxp3+ cells among CD3+ cells from spleen, lung, ...
<p>C57BL/6 and CBA/J mice were infected with an aerosolized dose of <i>Mtb</i> and at various timepo...
<p>(A) Purified SP4 thymocytes, and CD4<sup>+</sup> naïve T cells from lymph nodes of C57BL/6 mice w...