<i>ju1279</i> and <i>ju993</i> are novel alleles of <i>dhc-1</i> and <i>dnc-4</i>, respectively.

<p>(A) Gene structure of <i>dhc-1</i>, with <i>ju1279</i> and reference alleles <i>or195</i> and <i>js319</i> marked. <i>ju1279</i> alters the N-terminal Heavy chain domain 1, <i>or195</i> alters the coiled coil stalk and <i>js319</i> alters region D6 of the motor. (B) Representative images of DD synapses along the DNC (<i>P</i>_{<i>flp-13</i>}-SNB-1::GFP (<i>juIs137</i>)) in adult animals. Ex-DHC-1 denotes extrachromosomal copies of wild type DHC-1. Scale bar: 10 μm. (C) Quantification of synaptic puncta in the DNC of adult animals. Data are mean ± SEM; n>10 animals per genotype. Statistics: One-Way ANOVA followed by Tukey’s posttest; ***p<0.001, ns- not significant. (D) Gene structure of <i>dnc-4</i>, with <i>ju933</i> and the reference allele <i>or633</i> also marked. (E) Quantification of synaptic puncta in the DNC (<i>P</i>_{<i>unc-25</i>}-SNB-1::GFP (<i>juIs1</i>)) of adult animals. Ex-DNC-4(+) denotes extrachromosomal copies of wild type DNC-4. Data are mean ± SEM; n>8 animals per genotype. Statistics: One-Way ANOVA followed by Tukey’s posttest; ***p<0.001, ns- not significant. (F) Quantification of synaptic puncta in the DNC (<i>P</i>_{<i>flp-13</i>}-SNB-1::GFP (<i>juIs137</i>)) of adult animals. Animals were cultured at two different temperatures, the permissive (20^°C) and restrictive (25^°C) temperatures for <i>or633</i>, starting from late L1. Data are mean ± SEM; n>8 animals per genotype. Statistics: One-Way ANOVA followed by Tukey’s posttest; ns- not significant.