Identification of the formulation with the best knockdown efficiency by western blotting following nanoparticle transfection with different formulation (A. T1 and T2; B. T3-T8).

<p>318–1 cells were transfected with different formulations encapsulating either three different concentrations of p53 siRNA (<i>i</i>.<i>e</i>. 100, 125 and 150 pmol) (Fig 3A) or 150 pmol siRNA (Fig 3B). Representative western blotting results for p53, actin and vinculin are shown. This experiment was performed 3 independent times.