Phalloidin (TRITC) and antibody (FITC) staining of actin in <i>Siedleckia nematoides</i> before and after application of cytoskeletal drugs.

<p><b>A-D. Non-treated parasites: A-B.</b> Localisation of F-actin with phalloidin in parasites attached to the host tissue. <b>C.</b> Double labelling with phalloidin and specific anti-actin antibody. <b>D.</b> Double labelling with phalloidin (left) and anti-actin antibody (right), image split into two separate channels. <b>E-F. Double labelling with phalloidin (left) and anti-actin antibody (right) in parasites treated with JAS, images split into two separate channels: E.</b> 10 μM JAS (8 h). <b>F.</b> 30 μM JAS (6 h). <b>G-H. Double labelling with phalloidin (left) and anti-actin antibody (right) in parasites treated with cytochalasin D, images split into two separate channels: G.</b> 10 μM cytochalasin D (9 h). <b>H.</b> 30 μM cytochalasin D (8 h). A-B, D-H left: CLSM, phalloidin-TRITC/Hoechst; C: CLSM, IFA/phalloidin-TRITC/Hoechst; D-H right: CLSM, IFA/Hoechst; A-H: PFA fixation. <i>black arrowhead–</i>parasite caudal end, <i>black asterisk</i>–parasite apical end, <i>h–</i>host tissue.