Dietary obesity in mice with <i>Adipoq</i>-cre mediated inactivation of <i>Mest</i>.

<p>(A) <i>Mest</i> mRNA expression in inguinal (iWAT) and epididymal (eWAT) white adipose tissue of wildtype (WT; n = 9), WT.<i>Adipoq</i>-cre (WT-cre; n = 5), paternal floxed <i>Mest</i> (pFL; n = 11) and pFL.<i>Adipoq</i>-cre (ApKO; n = 17) mice after being fed a HFD from 8–24 weeks of age. <i>Mest</i> mRNA expression measured by TaqMan QRT-PCR is represented as the mean ± SEM of arbitrary units (AU) normalized to TATA-box binding protein (<i>Tbp</i>). Significance in <i>Mest</i> RNA expression between groups was determined via one-way ANOVA using Tukey’s multiple comparisons test. (B) Data shows the longitudinal measurements of bodyweight (BWT) for WT (n = 9), WT-cre (n = 5), pFL (n = 11) and ApKO (n = 17) mice fed a HFD from 8 to 24 weeks of age as indicated by the arrow along the X-axis. (C) Longitudinal measurements of fat mass (g) and (D) fat-free mass (g) measured by DEXA at the times indicated on the X-axis are shown for WT (n = 9), WT-cre (n = 5), pFL (n = 11) and ApKO (n = 17) mice fed a HFD from 8 to 24 weeks of age as indicated by the arrow along the X-axis. (B-D) All data in the longitudinal studies are presented as the mean ± SEM. Significance at each time point of the longitudinal phenotypic analyses was determined by 2-way ANOVA and Tukey’s multiple comparisons test. Time points annotated with ‘a’ and ‘b’ indicates significant differences between ‘ApKO vs pFL’ and ‘ApKO vs WT’.