Additional file 3 of Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130

Figure S1. gp130 expression following acute retinal detachment (RD) and isotype control experiment. (A) The gp130 expression (red) and Müller cells (CRALBP, green) in normal and detached retinas (bar = 50 μm). Two nuclei layers were stained with DAPI (blue). Retinal gp130 expression was initially confined to outer nuclear layer (ONL) in normal retina, and extended into the inner nuclear layer and monocytes in vitreous humor after RD. (B) Recruited monocytes floated in the vitreous space one day after RD (bar = 10 μm). CD11b (green) and membrane-bound gp130 (red) co-stained in monocytes. Nuclei were stained with DAPI (blue). (C) Additional evidence to support the therapeutic effect of anti-gp130 antibody. Intravitreal injection of an isotype control antibody (n = 3, p < 0.01) or PBS vehicle alone (n = 4, p < 0.001) immediately after RD did not reduce monocyte infiltration like injection of the anti-gp130 antibody (n = 4). (JPG 9157 kb