Characterization of differently tagged OATP1B3 proteins.

<p>(A) Uptake of 10 μM estrone-3-sulfate (E3S) or 1 μM estradiol-17-β-glucuronide (E17βG) was measured at 37°C with HEK293 cells transiently transfected with empty vector or OATP1B3 with a His-, HA- or FLAG-tag for 1 minute. Values are means ± SD of three independent experiments each performed with triplicate measurements. (B) HEK293 cells transiently transfected with OATP1B3 with a His-, HA- or FLAG-tag were fixed in the absence or presence of 1% TX-100. The different OATP1B3s were detected using the respective antibodies (shown in green) and nuclei were stained by DAPI (shown in blue).