<p>Confocal stacks after subcutaneous infection with <i>B</i>. <i>stabilis</i> in <i>mpx</i>:<i>GFP</i> embryos (STD intensity projection, 2 μm x 19 steps; T = 35–37 in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006437#ppat.1006437.s012" target="_blank">S2 Movie</a>). Arrows, rounded neutrophils (green) with vacuoles full of bacteria (red) at 64 minutes post injection (mpi) eject their cell contents in the surroundings (arrow heads 65.5 mpi), leaving bacterial clusters and cell debris (arrow heads, 66 min). Lower panels, MAX intensity projection of three consecutive slices (2 μm) at 65.5 mpi showing ejected cellular contents (diffuse GFP signal). Scale bars, 50 μm.</p
<p>(A) Double transgenic <i>Tg(mpeg</i>:<i>dendra2)</i> x <i>Tg(mpx</i>:<i>mCherry)</i> larvae show ...
<div><p>(A) Enumeration of infected macrophages in embryos by fluorescent and DIC microscopy after i...
<p>(A and B) WT or <i>tnfr</i> morphants were <i>iv</i> infected with R <i>Mabs</i> (tdTomato, ≈150 ...
<p><i>Tg(mpx</i>:<i>GFP)</i> embryos were injected at the one cell stage with either <i>csf3r</i> mo...
<p>(A) <i>Tg(mpx</i>:<i>GFP)/mpx</i><sup><i>+/-</i></sup> (A1, A2) and <i>tg(mpx</i>:<i>GFP)/mpx</i>...
<p>(A-B-C-D) <i>Tg(mpx</i>:<i>Gal4/UAS</i>:<i>nfsB-mCherry/mpeg1</i>:<i>GFPcaax)</i> embryos were tr...
<p>(A-D) Two dpf <i>tg(mpx</i>:<i>GFP)</i> embryos were either injected with PBS (A) or infected wit...
<p><b>(A)</b> Embryo survival (left; representative experiment) and bacterial burden (total of 2 exp...
<p>(A) Neutrophils at the IS at different time points. A diagram summarizing the events per time poi...
<p>(A-C) <i>Tg(mpx</i>:<i>GFP)</i> embryos were injected at the one cell stage with either <i>p47</i...
<div><p>(A and B) Embryos with aggregates at 3d postinfection with 85 CFU red-fluorescent WT bacteri...
<p>A–E: Frames extracted from <i>in vivo</i> time-lapse confocal imaging sessions of 3 dpf larvae in...
Impaired neutrophil recruitment upon SINV-local Shigella co-infection. Mpx:GFP larvae were injected ...
<p>(A, B) Confocal imaging of leukocytes and calcofluor-stained acuD:RFP fungal conidia in <i>Tg(mpx...
<p><b>(A,B)</b> Embryo survival (average inoculum 23 CFU, representative experiment) <b>(A)</b> and ...
<p>(A) Double transgenic <i>Tg(mpeg</i>:<i>dendra2)</i> x <i>Tg(mpx</i>:<i>mCherry)</i> larvae show ...
<div><p>(A) Enumeration of infected macrophages in embryos by fluorescent and DIC microscopy after i...
<p>(A and B) WT or <i>tnfr</i> morphants were <i>iv</i> infected with R <i>Mabs</i> (tdTomato, ≈150 ...
<p><i>Tg(mpx</i>:<i>GFP)</i> embryos were injected at the one cell stage with either <i>csf3r</i> mo...
<p>(A) <i>Tg(mpx</i>:<i>GFP)/mpx</i><sup><i>+/-</i></sup> (A1, A2) and <i>tg(mpx</i>:<i>GFP)/mpx</i>...
<p>(A-B-C-D) <i>Tg(mpx</i>:<i>Gal4/UAS</i>:<i>nfsB-mCherry/mpeg1</i>:<i>GFPcaax)</i> embryos were tr...
<p>(A-D) Two dpf <i>tg(mpx</i>:<i>GFP)</i> embryos were either injected with PBS (A) or infected wit...
<p><b>(A)</b> Embryo survival (left; representative experiment) and bacterial burden (total of 2 exp...
<p>(A) Neutrophils at the IS at different time points. A diagram summarizing the events per time poi...
<p>(A-C) <i>Tg(mpx</i>:<i>GFP)</i> embryos were injected at the one cell stage with either <i>p47</i...
<div><p>(A and B) Embryos with aggregates at 3d postinfection with 85 CFU red-fluorescent WT bacteri...
<p>A–E: Frames extracted from <i>in vivo</i> time-lapse confocal imaging sessions of 3 dpf larvae in...
Impaired neutrophil recruitment upon SINV-local Shigella co-infection. Mpx:GFP larvae were injected ...
<p>(A, B) Confocal imaging of leukocytes and calcofluor-stained acuD:RFP fungal conidia in <i>Tg(mpx...
<p><b>(A,B)</b> Embryo survival (average inoculum 23 CFU, representative experiment) <b>(A)</b> and ...
<p>(A) Double transgenic <i>Tg(mpeg</i>:<i>dendra2)</i> x <i>Tg(mpx</i>:<i>mCherry)</i> larvae show ...
<div><p>(A) Enumeration of infected macrophages in embryos by fluorescent and DIC microscopy after i...
<p>(A and B) WT or <i>tnfr</i> morphants were <i>iv</i> infected with R <i>Mabs</i> (tdTomato, ≈150 ...