Biofilm growth comparison.

<p>Stacked z-series of biofilms from various time-points stained with LIVE/DEAD stain (green/red). (A) 1291wt biofilms grown for 6, 24, or 48 h. (B) 1291<i>ackA</i> biofilms grown for 6, 24, or 48 h. These images show that 1291wt is able to form a confluent biofilm that remains viable at 48 h. In contrast, the 1291<i>ackA</i> mutant is initially able to form a biofilm, but by 24 h it is no longer viable. (C) The volume of the biofilms were also determined and analyzed using a Mann-Whitney comparison. A significant difference between overall size of the 1291wt and 1291<i>ackA</i> biofilms were seen at 24 h and 48 h (with p-values of 0.048 and 0.016, respectively). Biofilm experiments were performed in duplicate in two independent experiments.