Qualitative and Quantitative Analysis of Proteome and Peptidome of Human Follicular Fluid Using Multiple Samples from Single Donor with LC–MS and SWATH Methodology
- Aleksandra E. Lewandowska (4235956)
- Katarzyna Macur (1517119)
- Paulina Czaplewska (4235953)
- Joanna Liss (698689)
- Krzysztof Łukaszuk (698687)
- Stanisław Ołdziej (1522885)
DOIAbstract
Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small-scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient-specific. Ultrafiltration was used to fractionate hFF to high-molecular-weight (HMW) proteome (>10 kDa) and low-molecular-weight (LMW) peptidome (<10 kDa) fractions. HMW and LMW compositions were analyzed using LC–MS in SWATH data acquisition and processing methodology. In total we were able to identify 158 proteins, from which 59 were never reported...
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