Additional file 2: Figure S1. of PLX8394, a new generation BRAF inhibitor, selectively inhibits BRAF in colonic adenocarcinoma cells and prevents paradoxical MAPK pathway activation

The effect of vemurafenib and PLX8394 on the BRAF V600E melanoma cell line LM-MEL-64 and the BRAF wt melanoma cell line LM-MEL-39. (A) LM-MEL-64 and (B) LM-MEL-39 were treated with the indicated concentrations of vemurafenib or PLX8394 and immunoblotting for total and phosphorylated ERK was performed. (C) pERK densitometry relative to control expressed as (%) ± SD for LM-MEL-64 and (D) for LM-MEL-39. Data are from three independent experiments. (TIFF 342 kb