Expression evaluations of created pools by ELISA assay.

<p>Expression levels of all the created pools evaluated by ELISA assay while quantitative real-time PCR was employed to determine gene copy numbers. Non-transposed cells had similar gene copy number although N-pBLIH cells had—low expression level. Transposition based cells had similar copy numbers as well, however, their expression levels were also more similar. Error bars indicate SD of triplicate measurements. ANOVA was used to detect statistically significant differences between the generated pools (P< 0.05).