TLR3 and TLR2 Mediate B cell Response to poly I:C and Pam3CSK4 respectively.

<p>B cells purified from TLR2^-/- (A, C, E) or TLR3^-/- (B, D, F) mice were stimulated with poly I:C (25 μg/mL), Pam3CSK4 (1 μg/mL), the combination of poly I:C and Pam3CSK4, LPS (10 μg/mL) or CpG (25 μg/mL), with T-cell-dependent co-stimulation provided by anti-CD40 and anti-Ig. Relevant wild-type controls were tested in parallel (C57BL/6 for TLR2^-/-, B6;129SF2/J for TLR3^-/-). (A, B) Expression of CD80 was measured by flow cytometry after 24 hours. (C, D) Proliferation was measured by [³H]-TdR uptake after 3 days. (E, F) Production of TNF-α was measured by ELISA the supernatants harvested at 24 hours. Results are shown as the average ± SEM; TLR2^-/- is from 4 individual B cell preparations pooled from two independent experiments; TLR3^-/- is from 5 individual B cell preparations pooled from three independent experiments. Statistics compare the response of knockout B cells to corresponding wild type B cells and were calculated by 2-way ANOVA with Bonferroni post-test, *p<0.05, ***p<0.001.