Histological and RNA <i>in situ</i> hybridisation findings in the spleen, testes and heart of ZIKV-challenge A129 mice.

<p>(A) Spleen. Poorly defined areas comprising large mononuclear cells within the white pulp (Animal 86783, 10⁶ pfu ZIKV^AF, day 5). Inset, normal spleen with well defined, small germinal centres within the white pulp (Animal 86739, unchallenged). (B) Spleen. Prominent, extra-medullary haematopoiesis in the red pulp. Rectangle, numerous PMNs in the red pulp sinuses (Animal 86724, 10⁶ pfu ZIKV^AF, day 7). (C) Testis. Expansion of the interstitial tissue by proteinaceous fluid, macrophages and PMNs. Inset, higher power image of area within white square (Animal 86772, 10 pfu ZIKV^AF, day 5). (D) Testis. Mild infiltration of PMNs into the interstitial space with positive viral staining (Animal 86779, 10⁶ ZIKV^AF, day 3). (E) Testis. Positive staining of virally infected cells focally within the walls of the seminiferous tubules (white arrows) as well as within the interstitium (Animal 86784, 10 pfu ZIKV^AF, day 7). (F) Testis. Epididymis with positive staining of cells in lumen and epithelium of the efferent ductules as well as the interstitium (Animal 86784, 10 pfu ZIKV^AF, day 7). (G) Testis. Positive staining of cells in the necrotic seminiferous tubules (Animal 86744, 10⁶ pfu ZIKV^AS, day 14). (H) Testis. Intra-tubular and interstitial cell staining (Animal 86757, 10⁶ pfu ZIKV^AS, day 7). (I) Heart. Infiltration of myocardium by macrophages and PMNs (Animal 86779, 10⁶ ZIKV^AF, day 3). (J) Heart. Infiltration of an atrio-ventricular valve by macrophages and PMNs (Animal 86780, 10⁶ pfu ZIKV^AF, day 3). A-C and I-J show sections stained with haematoxylin and eosin (H&E) and D-H show RNA <i>in situ</i> hybridisation images.