Neurite alignment on nanofiber mats <i>in vitro</i>.

<p>(A–C) Representative images of TUJ1 stained terminally differentiated H7 hESC derived neurons (green) on PS coverslips (A) and unaligned (B) and aligned (C) nanofiber mats. Insets in B’ and C’ show light microscopic images of unaligned and aligned nanofiber mats. (D–F) Plot of image intensity as a function of angle with corresponding FWHM values after Fourier transformation of fluorescence images of A, B and C illustrating the extent of alignment. A low FWHM indicates high alignment. (G) Quantification of FWHM values of coverslips (n = 8) and unaligned (n = 7) and aligned (n = 6) fibers terminally differentiated for 2–6 weeks. For random samples, only one out of 7 samples allowed FWHM calculation. (H) Immunohistochemistry for TUJ1 (green) and VGLUT1 (red) shows that neurons maintain a glutamatergic fate on fiber mats. Scale bars represent 100 μm in A, B, C and H and 50 μm in B’ and C’.