MOESM3 of A novel Sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm

Additional file 3: Figure S2. Map of promoter:gene constructs used for sugarcane transformation. SCBV21: Sugarcane bacilliform virus promoter; Ubi1: Maize ubiquitin 1 promoter; Pr4: Ubi1 promoter without heat-shock elements (5′-TGGACCCCTCTCGAGAGTTCCGCTC-3′); E35S: Enhanced Cauliflower mosaic virus (CaMV) 35S (2×35S) promoter; EYFP: enhanced yellow fluorescent protein gene; GUS: β-glucuronidase gene; Nos: Agrobacterium tumefaciens nopaline synthase terminator. Ubi1:GUS (pAHC27 vector) [3] and CaMV 35S:GUS (pBI221 vector) (Clontech, Takara Bio USA, Inc., Mountain View, CA, USA) are used. B, BamHI; Bb, BbsI; E, EcoRI; H, HindIII; N, NcoI; P, PstI; Sa, Sall; Sc, SacI; Sm, SmaI; Sp, SphI; X, XbaI; Xh, XhoI; and Xm, XmaI. Unique enzyme sites are indicated in red. Boxes are not drawn to scale. A triangle represents the deletion of heat-shock elements (25 bp) in the Ubi1 promoter