MOESM1 of A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga, Nannochloropsis oceanica

Additional file 1: Figure S1. The ultrastructure of N. oceanica cells under nitrogen-replete (left) and nitrogen-depleted (right) conditions. Figure S2. GC-MS chromatogram of fatty acids in the sn-2 and sn-1/sn-3 positions of TAG from N. oceanica cells under nitrogen-replete (+N) and nitrogen-depleted (-N) conditions. Figure S3. Cladogram of the DGATs from plants, fungi, algae and animals. The neighbor-joining method was used to reconstruct the cladogram using MEGA6 [34], with the bootstrap value (obtained from 1000 replicates) is shown on each node. The scale bar 0.1 represents 10% divergence, calculated as the estimated number of replacement. Circles, plants; Squares, fungi; Filled circles, algae; triangles, animals. Protein sequences used for the cladogram construction see Additional file 2: Table S1. Figure S4. Predicated transmembrane domains for NoDGAT1A NoDGAT1B by TMHMM (V2.0, http://www.cbs.dtu.dk/services/TMHMM/). Figure S5. Protein sequence alignment of putative DGAT1s. The alignment was conducted using ClustalX2.1. The sequences used see Additional file 2: Table S1. Red arrows indicate the key amino acid residues identified by previous studies. Figure S6. The transcriptional expression levels of NoDGAT1A and NoDGAT1B in H1246 (upper) and INVSc1 (lower), as determined by quantitative real-time PCR. The gene expression levels were normalized to the endogenous ACT1 gene. Figure S7. TAG content per dry weight (a) and the ratio of fatty acids in TAG over TFA (b) in NoDGAT1A knockdown lines and EV. Algal cells grown in nitrogen-replete medium for 4 days (considered as 0 h of nitrogen depletion) were used for nitrogen depletion experiment. Data are expressed as mean ± SD (n=3). Asterisks indicate the significant difference compared with EV (t-test, P<0.05). Figure S8. TAG content per dry weight (a) and the ratio of fatty acids in TAG over TFA (b) in NoDGAT1A overexpression lines and EV. Algal cells grown in nitrogen-replete medium for 4 days (considered as 0 h of nitrogen depletion) were used for nitrogen depletion experiment. Data are expressed as mean ± SD (n=3). Asterisks indicate the significant difference compared with EV (t-test, P<0.05). Figure S9. TAG content per dry weight (a) and the ratio of fatty acids in TAG over TFA (b) in NoDGAT1A heterologous expression lines of Chlamydomonas and EV. Algal cells grown in nitrogen-replete medium for 4 days (considered as 0 h of nitrogen depletion) were used for nitrogen depletion experiment. Data are expressed as mean ± SD (n=3). Asterisks indicate the significant difference compared with EV (t-test, P