tRNA transfection accumulates sarkosyl insoluble tau in human-induced pluripotent stem cell (hiPSC) derived neurons.

<p>(A) Representative western blot of neurons harboring a P301L <i>tau</i> mutation transfected with tRNA, but not cells transfected in the absence of nucleic acids (Mock) present evidence for accumulation of sarkosyl insoluble tau (Ins.) as seen in the intense band in lane 9 labeled with PHF-1 antibody. Addition of tRNA to the lysis buffer is insufficient to increase the tau present in the insoluble fractions (compare Mock + tRNA, lane 6 to tRNA, lane 9). (B-C) Quantification of PHF-1 tau and β-actin level for both neurons harboring a P301L tau mutation (B) and neurons expressing wild type tau (C) are shown. Error bar represents standard error of the mean. * <i>p</i> < 0.05, *** <i>p</i> < 0.001, <i>n</i> > 3. The numerical data used in B and C are included in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2002183#pbio.2002183.s010" target="_blank">S1 Data</a>.