DNA damage in Panc-1 cells.

<p>(A) Pan-nuclear γH2AX signals of Panc-1 cells normalized to values of mock treated cells at time points 1 h and 12 h after treatments as surrogates for DNA double strand breaks (n = 3). SFN-treated cells were incubated with either 20 μM or 40 μM SFN for 24 h and afterwards if indicated irradiated with 4 Gy at time point 0 h. (B+C) Western blot of NHEJ pathway proteins after treatment of Panc-1 cells with SFN for 24 h and statistics of their band densities normalized to the loading control. For statistics data of Students t-test are shown (* p<0.05, ** p<0.01, *** p<0.001).