Gal-9 inhibits neutrophil chemotaxis, an effect that is reversed by the addition of lactose.

<p>The effect of 4-h preincubation of 10-nM Gal-9 (closed bar) or HBSS (open bar) as a control on neutrophil chemotaxis in response to KC (20 ng/mL) was examined in 48-well chemotaxis chambers. Gal-9 significantly reduced chemotaxis and the addition of lactose reversed this chemotaxis inhibition. Data are representative and are represented as the mean neutrophil count per high-powered field at 400× magnification (Number of cells/HPF) ± SEM. n = 5–8. *p < 0.05; **p < 0.01. Abbreviations: Gal-9, galectin-9; HBSS, Hanks’ balanced salt solution; KC, keratinocyte-derived cytokine; HPF, high power field; SEM, standard error of the mean.