Recognition of purified, recombinant LigA7’-13 and LigB0-7 proteins by pooled sera from immunized hamsters.

<p>One to two hundred nanograms per well of purified His-tagged LigA7’-13 (A) or LigB0-7 (B) were run in 4–12% SDS-PAGE then transferred to PVDF membrane for western blot analysis. The membrane was cut to strips and probed with 1:5,000 pooled sera collected before immunization (pre-bleed, P) and at day 32 (last bleed, LB) from immunized and control groups. Another membrane strip was incubated with 1:5,000 rabbit α-Lig as positive control (Co). Sera collected from LigA7’-13-vaccinated animals cross reacted with recombinant LigB0-7 and vice versa.