Effects of embelin on proliferation and cell cycle progression in leukemic cells.

<p>Embelin inhibits the cell viability of leukemic cells. K562 <b>(A)</b> and U937 <b>(B)</b> cells were incubated with embelin as indicated in the figure for 24 h. Cell proliferation assays were performed using MTT as described in Materials and Methods. The graph displays the mean +/- SD (standard deviation) of three independent experiments. *<i>P</i><0.001 Cell cycle fractions of embelin treated leukemic cell lines. K562 <b>(C)</b> and U937 <b>(D)</b> cells were treated with embelin as indicated in figure for 24 h. Thereafter, the cells were stained with Hoechst 33342, and analyzed for DNA content by flow cytometry as described in Materials and Methods. A representative of three independent experiments is depicted in the figure. Embelin significantly increased subG0 population in K562 <b>(E)</b> and U937 <b>(F)</b> cells. The graph displays the mean +/-SD of three independent of experiments *<i>P</i> < 0.001.