Microfluidic assay of circulating endothelial cells in coronary artery disease patients with angina pectoris - Fig 3

<p>(<b>A</b>) Specially designed microfluidic system for CECs isolation: (1) the schematic diagram of blood sample processing; (2) SEM photograph of staggered capture units; (3) Microfluidic chip [(i) sample inlet, (ii) buffer inlet, (iii) waste outlet]; (4) Setup of microfluidic system [(i) blood sample reservoir, (ii) buffer reservoir, (iii) waste reservoir driven by a syringe pump]. (<b>B</b>) Immunofluorescent labeling of isolated HUVECs: HUEVECs (DAPI⁺/CD146⁺/VEGFR1⁺/CD45^-) are marked by green arrows, residuary leukocytes (DAPI⁺/CD45⁺) are marked by red arrows; Scale bar, 50μm.