Comparison of the frequencies of KIR3DL01⁺ and KIR3DL05⁺ NK cells in lymph nodes and gut-associated lymphoid tissues.

<p>The frequencies of KIR3DL05⁺ (A), KIR3DL01⁺ (B) and KIR3DL01^-05^- (C) NK cells in PBMCs, lymph nodes (LN) and gut-associated lymphoid tissues (GALT) were compared prior to SIV infection (week -4) and at weeks 2 and 8 after SIV infection. The frequencies of CD16⁺, CD56⁺ and CD16^-CD56^- NK cells in lymph nodes (D & E) and gut-associated lymphoid tissues (F & G) expressing KIR3DL05 (D & F) or KIR3DL01 (E & G) were also compared at weeks -4, 2 and 8 pre- and post-infection. Representative gating for differentiating KIR3DL01⁺ versus KIR3DL05⁺ NK cells in lymphocytes isolated from colorectal biopsies is illustrated in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006506#ppat.1006506.s007" target="_blank">S7 Fig</a>. Significant differences are indicated with asterisks (p<0.05*, p<0.01**, p< 0.005*** & p<0.001****, Mann-Whitney <i>U</i>-test). Samples with less than 30 events per gate were excluded from the analysis.