Evaluation of resazurin microtiter plate assay and HPLC- photodiode array analysis of the roots of <i>Asparagus adscendens</i>

<p><i>Asparagus adscendens</i> Roxb. (Asparagaceae), is native to the Himalayas<b>.</b> The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of <i>A. adscendens</i> and its solid-phase extraction (SPE) fractions using resazurin microtitre assay against Gram-positive and negative bacterial-registered strains and to carry out HPLC-photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5–0.009 mg/mL) than against Gram-negative bacteria (MIC: 1.25–2.5 mg/mL). The use of microtitre plates has the advantage of lower cost, fast and quantitative results. Like other <i>Asparagus</i> species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.