RNA binding and translation inhibition by the CP^WT and CP^RA proteins.

<p>(A) RNA-binding abilities of the GST-CP^WT and GST-CP^RA proteins as assessed by digoxigenin-labeled CMV RNA4 or Luciferase (Luc) mRNA (See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006522#sec011" target="_blank">Materials and methods</a> for details). GST served as a negative control. Coomassie brilliant blue (CBB) staining was used as the protein loading control. (B) Translation inhibition of Luciferase mRNA by CP^WT and CP^RA <i>in vitro</i>. The Luc mRNA was transcribed and incubated with different concentrations of the GST, GST-CP^RA, or GST-CP^WT in wheat germ extract at 25°C for two hours. Then, the activity of translated Luciferase <i>in vitro</i> was measured with a luminometer. Luciferase activities from mRNA without GST, GST-CP^RA, or GST-CP^WT was set as 100%. Error bars represent the standard error of the mean. Data points are the mean value of three independent experiments. *<i>P</i>-value < 0.05; ** <i>P</i>-value < 0.01; *** <i>P</i>-value < 0.001.