Additional file 5: of Ischaemic stroke and the recanalization drug tissue plasminogen activator interfere with antibacterial phagocyte function

Influence of adrenaline, noradrenaline, dexamethasone and acetylcholine on mean NET area. Effect of in vitro administration of 1 × 10−7 M or 1 × 10−5 M adrenaline (A) and noradrenaline (B); 2.5 × 10−7 M or 2.5 × 10−6 M dexamethasone (C); and 5.5 × 10−6 M or 5.5 × 10−4 M acetylcholine (D) on NET area was tested. After incubation of blood of healthy donors with hormones and acetylcholine for 4 h at room temperature, neutrophils were isolated, and NETs were induced using PMA or fMLP or left unstimulated (nA,B = 7; nC,D = 6). *p < 0.05. The mean and SD ranges are given. ANOVA and Bonferroni comparisons as post hoc tests were used. Repeated measures tests were used within each of the three stimulation conditions. (PDF 2057 kb