<i>Salmonella</i> typhimurium and <i>Salmonella</i> typhi stimulate specific gene expression patterns in cultured epithelial cells.

<p>(<b>A</b>) Venn diagram depicting the number of unique and common genes whose expression changed at least 3 fold at the indicated times after infection with <i>S</i>. Typhimurium (Tm) or <i>S</i>. Typhi (Ty). (<b>B</b>) Heat map of genes whose expression changed at least 8 fold at the indicated times after infection with wild type <i>S</i>. Typhimurium (Tm) or <i>S</i>. Typhi (Ty). The data are presented as row-normalized heat maps as indicated in the color scale. * p < 0.04, Student t test. (<b>C</b>) Expression of selected genes in infected Henle-407 cells at the indicated times after infection. Values (mean ± SD of 3 replicates) represent the GAPDH normalized transcript levels of selected genes in <i>S</i>. Typhimurium (Tm) or <i>S</i>. Typhi (Ty) infected cultured Henle-407 cells relative to levels of uninfected cells. (<b>D</b>) Western blot detection of selected proteins (SerpinB3 and TTP) in infected Henle 407 cells at the indicated time after infection with <i>S</i>. Typhimurium (Tm) or <i>S</i>. Typhi (Ty). Levels of tubulin in the different samples served as loading controls. (<b>E</b>) Immunofluorescence staining of endogenous SerpinB3 in Henle-407 cells after infection with <i>S</i>. Typhimurium (Tm) or <i>S</i>. Typhi (Ty). Cells were stained at the indicated times after infection with antibodies directed to SerpingB3 (green) or bacterial LPS (red), and DAPI (blue) to detect DNA.