Immunofluorescence double staining assay on GIM biopsies of HCV-transplanted patients.

<p>Immunofluorescence assay on colon polyps of HCV-transplanted patients (<i>n</i> = 2), using antibodies against HCV core antigen (green) (A, D) and Chromogranin A (red) (B, E). Negative control was performed by omitting the primary antibodies and using polyclonal FITC-conjugated Donkey anti-goat (G) and Cy3-conjugated Donkey anti-mouse (H) as secondary antibodies. I, DAPI only. Nuclei were counterstained using DAPI. Scale bar: 20 μm. Double staining showed a clear positivity of enteroendocrine cells for HCV core Ag (merged C, F).