Immune-histochemical staining of HCV proteins on paraffin-embedded GIM biopsies sections of HCV-transplanted patients.

<p>Immune-histochemical staining of HCV proteins was performed on paraffin-embedded GIM biopsies sections of HCV-transplanted patients before (A-E) and after transplantation (F-L (Original magnification X400). (A, F) NS3 in stromal cells of duodenum polyps, (A patient 18, F patient 14); (B, G) NS4 in stromal cells of colon polyp (B patient 7, G patient 25); (C) E1 in stromal cells of colon (patient 18); (H) E1 in glandular epithelial cells of duodenum (patient 10); (D) E2 in glandular epithelial cells of rectum polyp (patient 9); (I) E2 in stromal cells of duodenum polyp (patient 14); (E) Core in glandular epithelial cells of duodenum (patient 15); (L) Core in glandular epithelial cells of cecum polyp (patient 10). Negative controls (M-Q) were performed by omitting the primary antibodies, and by using anti-mouse (M-O) and anti-goat (P, Q) secondary antibodies. (M) NS3 in antrum; (N) NS4 in polyp body; (O) E1 in antrum; (P) E2 in polyp colon; (Q) Core in polyp colon. Scale bar 50 μm.