<p>Tumor cells were co-cultured with fibroblasts for 5 days. Freshly isolated naïve monocytes were added to tumor cell/fibroblast co-culture on day 5 and cell viability was measured every 2 days from day 5 to 11. Addition of monocytes did not influence the co-culture´s viability and tumor cell/fibroblast co-cultures with monocytes were as viable as co-cultures without monocytes for each day and tumor cell line. Represented are n = 5 independent experiments. Using an unpaired Student´s <i>t</i> test with unequal variances, no significances were observed between tumor cell/fibroblast co-cultures with and without monocytes.</p
<p>Human monocytes were cultured in media alone, or with CMFDA-labeled MDA, or live mf of <i>Brugia ...
<p>SEM pictures were taken after ten days: Monocultures of A549 and SV80 were seeded in a ratio of 2...
Human peripheral blood monocytes secrete a soluble factor that enhances the ability of human epithel...
<p>Tumor cells and MRC5 fibroblasts were cultured as either co- cultures or monocultures as describe...
<p>To compare the 3D co-culture system to the 2D co-culture and trans- well co-culture systems, tumo...
<p>Fibroblasts were incubated with CFSE before initially seeding them together with tumour cells in ...
<p>One tumor cell line that exhibited the greatest fold-change in cell survival due to co-culturing ...
<div><p>In recent years, evidence has indicated that the tumor microenvironment (TME) plays a signif...
In recent years, evidence has indicated that the tumor microenvironment (TME) plays a significant ro...
<p>(A) The diagram of the co-culture protocol. NAF 200N.P7 co-cultured with MDA-MB-468 cells for fou...
nF or mF fibroblasts were seeded into the bottom of 6-well culture plates. Epithelial cells were cul...
<p>Mononuclear cells (MNC) were stimulated with phytohemaglutinin (PHA) and then cultured with or wi...
<p>MSC were seeded in equal numbers (1000 cells/cm<sup>2</sup>) on tissue culture dishes which were ...
The tumor microenvironment is a heterogeneous population of cells actively involved in the process o...
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. The tumor microenvironment is...
<p>Human monocytes were cultured in media alone, or with CMFDA-labeled MDA, or live mf of <i>Brugia ...
<p>SEM pictures were taken after ten days: Monocultures of A549 and SV80 were seeded in a ratio of 2...
Human peripheral blood monocytes secrete a soluble factor that enhances the ability of human epithel...
<p>Tumor cells and MRC5 fibroblasts were cultured as either co- cultures or monocultures as describe...
<p>To compare the 3D co-culture system to the 2D co-culture and trans- well co-culture systems, tumo...
<p>Fibroblasts were incubated with CFSE before initially seeding them together with tumour cells in ...
<p>One tumor cell line that exhibited the greatest fold-change in cell survival due to co-culturing ...
<div><p>In recent years, evidence has indicated that the tumor microenvironment (TME) plays a signif...
In recent years, evidence has indicated that the tumor microenvironment (TME) plays a significant ro...
<p>(A) The diagram of the co-culture protocol. NAF 200N.P7 co-cultured with MDA-MB-468 cells for fou...
nF or mF fibroblasts were seeded into the bottom of 6-well culture plates. Epithelial cells were cul...
<p>Mononuclear cells (MNC) were stimulated with phytohemaglutinin (PHA) and then cultured with or wi...
<p>MSC were seeded in equal numbers (1000 cells/cm<sup>2</sup>) on tissue culture dishes which were ...
The tumor microenvironment is a heterogeneous population of cells actively involved in the process o...
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. The tumor microenvironment is...
<p>Human monocytes were cultured in media alone, or with CMFDA-labeled MDA, or live mf of <i>Brugia ...
<p>SEM pictures were taken after ten days: Monocultures of A549 and SV80 were seeded in a ratio of 2...
Human peripheral blood monocytes secrete a soluble factor that enhances the ability of human epithel...