Development of callus and production of somatic embryos.

<p>(a) The leaf-to-callus transition on SH medium (21 days) from primary leaf explants of <i>Medicago truncatula</i> non-embryogenic genotype (M9) and variant with embryogenic phenotype (M9-10a). (b) Callus Relative Fresh Weight Growth (CRFWG) during induction phase of M9 and M9-10a explants. (c) Callus Relative Growth Rate (CRGR) during induction phase of M9 and M9-10a explants. (d) Somatic embryos production after three weeks of differentiation phase on MS differentiation medium. Statistical two-way ANOVA analysis with confidence interval 0.05 and Sidak post-hoc tests significance between groups indicated as two asterisks for P≤ 0.01 and four asterisks for P≤ 0.0001. Bars indicate +/- SD (n = 3). Scale bars 5 mm.